The two ChlorophytaScenedesmus abundans and Chlorella pyrenoidosa were collected from National Collection of Industrial Microorganism (NCIM), Pune, India. They were cultured in Fog’s media and experimental condition was maintained at temperature of 280C with 12:12 hrs. Photo and dark periods until stationary growth phase was reached. The cultures were harvested by centrifugation and the supernatant was discarded. The pellets were dried overnight in air oven at temperature of 400C. The dried powder was stored at airtight plastic container for subsequent nutritional analysis, for PUFA contents. The result of fatty acid composition showed that S. abundans had PUFA content of 7.19mg/g-1, GLA content of 21.30 mg/g-1, omega-3 content of 4.80mg/g-1, while omega -6 content of 3.60mg/g-1 in comparison with C. pyrenoidosa whosePUFA content was of 5.65mg/g-1, GLA content of 18.35 mg/g-1, omega-3 content of 2.90mg/g-1 and omega-6 content of 5.10mg/g-1. Thus the result showed both had great potential for nutritional benefits.